With the advent of genomic era, there have been systematic efforts to map gene expression profiles by in situ hybridization (ISH) and enhancer-trapping strategy.
In this article, we describe the methodology of these techniques, taking examples from our analyses of the mammalian cerebral cortex.(1998) suspected that the XNP protein is somehow involved in regulation of gene expression.Genetic and biochemical studies had led to the emerging concept that SNF2-like proteins are components of a large protein complex that may exert its functions by modulating chromatin structure. (1998) performed a yeast 2-hybrid analysis with XNP and several human heterochromatin-associated proteins.They found a specific interaction between XNP and the EZH2 (601573) proteins.In light of these observations, they discussed how the XNP protein may regulate gene transcription at the chromatin level. (1999) showed that ATRX protein is associated with pericentromeric heterochromatin during interphase and mitosis.
(1999) showed that the localization of ATRX to heterochromatin is encoded by its N-terminal region, which contains a conserved plant homeodomain-like finger and a coiled-coil domain.